Examen de Doctorado
Nancy Shyrley García Rojas
Les invitamos al examen final de la M. en C. Nancy Shyrley García Rojas para obtener el grado de Doctorado en Biotecnología de Plantas
🗓️ lunes 25 de agosto
⏰ 10:00 Seminario público
⏰ 14:00 Lectura del acta
📍 Auditorio Dr. Manuel Ortega, Unidad Irapuato
Biotecnología de Plantas | Cinvestav Irapuato
Examen para obtener el grado de Doctorado que presenta:
M. en C. Nancy Shyrley García Rojas
" Development of a Direct Mass Spectrometry Method for Disease Screening Using Dried Blood Spots"
Lunes 25 de agosto del 2025
10:00 horas SEMINARIO PUBLICO
14:00 horas LECTURA DEL ACTA Y AGRADECIMIENTOS
Auditorio Dr. Manuel Ortega Ortega
Unidad Irapuato
DIRECTORES DE TESIS
Dr. Robert Winkler
Dr. David S. Wishart
COMITÉ TUTORIAL
Dra. Gertrud Lund
Dr. Octavio Martinez de la Vega
Dra. Silva Valdés Rodriguez
Dra. Daniela Joyce Trujillo Silva
RESUMEN
Early disease detection through blood-based screening is critical for reducing morbidity and mortality. While liquid blood or serum samples are commonly used, their requirement for cold storage and transportation presents significant logistical and financial challenges. In contrast, dried blood or serum spots (DBS/DSS) on filter paper offer a cost-effective alternative by eliminating the need for refrigeration. Liquid chromatography–mass spectrometry (LC-MS) is the standard analytical method for identifying disease-related biomarkers in such samples; however, it is costly and labor-intensive due to extensive sample preparation. Ambient ionization mass spectrometry (AIMS), specifically Paper Spray (PS), enables direct sample analysis with minimal preparation, though it has primarily been used for detecting drugs in DBS/DSS and suffers from limitations in throughput and reproducibility. This project aimed to address these limitations by developing a high-throughput PS-MS platform. A custom 3D-printed cartridge plate accommodating 10 PS cartridges was designed, alongside a robotic system capable of processing up to 100 cartridges. The platform’s reproducibility was validated across two laboratories using different MS instruments. The study identified solvent and paper polarity as influencing factors in metabolite detection, with basic chemical groups—especially in acylcarnitines—being key to successful ionization. A robust detection method for acylcarnitines in serum was developed, and a patent application has been submitted. This work contributes a scalable, reproducible PS-MS method with potential application in large-scale disease screening.