Unravelling Vanillin Biosynthesis: Integrative Transcriptomic and Metabolomic Insights into Vanilla planifolia Pod Development
Artículo
Te invitamos a leer el artículo "Unravelling Vanillin Biosynthesis: Integrative Transcriptomic and Metabolomic Insights into Vanilla planifolia Pod Development" publicado en Journal of Agricultural and Food Chemistry, a cargo del investigador Cinvestav Dr. José Juan Ordaz Ortiz y su equipo de trabajo de la UGA, en conjunto con el investigador Cinvestav Dr. Luis Alfredo Cruz Ramirez.
Autores: Rebeca Hernández-Peña / José Luis Lorenzo-Manzanarez / Luis Alfredo Cruz-Ramírez / Delfino Reyes-López / Carmela Hernández-Domínguez / Fermín Pascual-Ramírez / José J. Ordaz-Ortiz
-
Metabolomics and Mass Spectrometry Group, Unidad de Genómica Avanzada, Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional, Irapuato, Gto. Mexico
-
Molecular and Developmental Complexity Group, Unidad de Genómica Avanzada, Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional Irapuato, Gto. Mexico
-
Facultad en Ciencias Agrícolas y Pecuarias, Benemérita Universidad Autónoma de Puebla, Puebla, Mexico
-
Instituto de Investigaciones en Ecosistemas y Sustentabilidad, UNAM, Campus Morelia, Morelia, Michoacán, Mexico.
Felicitamos al estudiantado y profesorado que contribuyeron en esta investigación por su arduo trabajo.
Summary:
Vanilla is the most popular flavor in the world and the second most valuable spice after saffron. Vanillin (4-hydroxy-3-methoxybenzaldehyde) is the predominant compound in natural vanilla flavor, a complex mixture comprising over 200 compounds. However, its biosynthetic pathway remains unclear. This study presents a comprehensive transcriptomic and metabolomic analysis of Vanilla planifolia leaves and pods throughout development, providing insights into vanillin biosynthesis. Differential gene expression analysis identified key genes involved in the ferulate and benzoate pathways, while metabolomic profiling revealed stage-specific accumulation patterns of pathway intermediates. Vanillin levels peaked 6 months after pollination, followed by a steady increase in the glucoside form. This integrative omics approach suggests a coordinated regulation of both pathways during fruit development, offering valuable insights into the metabolic dynamics of vanillin production and establishing a foundation for future research on genetic and metabolic engineering strategies in V. planifolia.