OsNLP3 and OsPHR2 orchestrate direct and mycorrhizal pathways for nitrate uptake by regulating NAR2.1–NRT2s complexes in rice
Artículo
Te invitamos a leer el artículo "OsNLP3 and OsPHR2 orchestrate direct and mycorrhizal pathways for nitrate uptake by regulating NAR2.1–NRT2s complexes in rice" publicado en PNAS, a cargo del profesor investigador Dr. Luis Rafael Herrera Estrella y su equipo de trabajo de la UGA.
Autores: Shuangshuang Wang / Hanghang Ye / Congfan Yanga / Yan Zhang / Yuhan Ren / Kun Xie / Lingxiao Wang / Dechao Zeng / Haoqiang He / Haoyan Ji / LLuis Herrera-Estrella / Guohua Xu / Aiqun Chen
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State Key Laboratory of Crop Genetics
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Department of Plant Nutrition
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College of Resources and Environmental Sciences
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Nanjing Agricultural University
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Nanjing 210095, China
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Unidad de Genomica Avanzada (UGA) del Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional (Cinvestav), Irapuato, Guanjuato, Mexico
Felicitamos al estudiantado y profesorado que contribuyeron en esta investigación por su arduo trabajo.
Summary:
Nitrogen (N) is the most important essential nutrient required by plants. Most land plants have evolved two N uptake pathways, a direct root pathway and a symbiotic pathway, via association with arbuscular mycorrhizal (AM) fungi. However, the interaction between the two pathways is ambiguous. Here, we report that OsNAR2.1–OsNRT2s, the nitrate (NO3−) transporter complexes with crucial roles in direct NO3− uptake, are also recruited for symbiotic NO3− uptake. OsNAR2.1 and OsNRT2.1/2.2 are coregulated by NIN-like protein 3 (OsNLP3), a key regulator in NO3− signaling, and OsPHR2, a major regulator of phosphate starvation responses. More importantly, AM symbiosis induces expression of OsNAR2.1–OsNRT2s, OsNLP3, and OsSPX4, encoding an intracellular Pi sensor, in arbuscular-containing cells, but weakens their expression in the epidermis. OsNAR2.1 and OsNLP3 can activate both mycorrhizal NO3− uptake and mycorrhization efficiency. Overall, we demonstrate that OsNLP3 and OsPHR2 orchestrate the direct and mycorrhizal NO3− uptake pathways by regulating the NAR2.1–NRT2s complexes in rice.