Effect of Hydrogen Sulfide on Sympathoinhibition in Obese Pithed Rats and Participation of K+ Channel
12 de octubre 2025
Te invitamos a leer el artículo: "Effect of Hydrogen Sulfide on Sympathoinhibition in Obese Pithed Rats and Participation of K+ Channel", realizado por el Dr. David Centurión, investigador de farmacología cardiovascular en Sede Sur del Cinvestav.
Autores: Carolina B. Gómez, Araceli Sánchez López, Karla Carvajal y David Centurión.
Abstrac: Elevated blood pressure is the leading metabolic risk factor in attributable deaths, and hydrogen sulfde (H2S) regulates vascular tone and blood pressure. Tus, this study aims to evaluate the mechanism by which NaHS (H2S donor) produces inhibition of the vasopressor sympathetic outfow in obese rats. For that purpose, animals were fed a high-fat diet (HFD) (60% calories from fat) for 12 weeks. Tey were anesthetized, pithed, and cannulated to evaluate the role of the potassium channel on NaHS-induced sympathoinhibition. Animals received selective electrical stimulation of the vasopressor sympathetic outfow, an intravenous (i.v.) administration of (1) tetraethylammonium (TEA, non-selective K+ channel blocker, 16.5 mg/kg), (2) 4-aminopyridine (4-AP, KV channel blocker, 5 mg/kg), (3) barium chloride (BaCl2, KIR channel blocker, 65 μg/kg), (4) saline solution (vehicle of TEA, 4-AP, and BaCl2, 1 mL/kg), (5) glibenclamide (KATP channel blocker, 10 mg/kg), and (6) glibenclamide vehicle (DMSO + glucose 10% + NaOH, 1 mL/kg), and then a 310 μg/kg·min NaHS i.v. continuous infusion. We observed that (1) NaHS produced inhibition of the vasopressor sympathetic outfow and (2) the sympathoinhibitory efect by NaHS was reversed by the KIR channel blocker, BaCl2, in obese rats. Te above data suggest that the potassium channel could be involved in the sympathoinhibition induced by NaHS.
Keywords: Blood pressure; cardiovascular; high-fat diet; hydrogen sulfde; potassium channel.