Resistance Assessment to PHYVV and PepGMV in Wild and Domesticated Accessions of Capsicum annuum L. by Bioballistic Inoculation
Karla Vanessa De Lira Ramos, Ernesto González Gaona, José Francisco Morales Domínguez, Diana Lilia Trejo Saavedra, Joaquín Sosa Ramírez, Rafael F. Rivera Bustamante and José de Jesús Luna Ruíz
Te invitamos a leer el artículo "Resistance Assessment to PHYVV and PepGMV in Wild and Domesticated Accessions of Capsicum annuum L. by Bioballistic Inoculation" publicado en "plants" en el que colaboró Diana Lilia Trejo Saavedra de Cinvestav Irapuato.
Autores:
Karla Vanessa De Lira Ramos, Ernesto González Gaona, José Francisco Morales Domínguez, Diana Lilia Trejo Saavedra, Joaquín Sosa Ramírez, Rafael F. Rivera Bustamante and José de Jesús Luna Ruíz
Resumen:
Chili pepper (Capsicum annuum L.) is an economically important crop in Mexico, with a production that is limited by viral diseases caused by Begomovirus infections such as PHYVV and PepGMV, both transmitted by Bemisia tabaci. These viruses affect both domesticated cultivars and wild populations. The use of resistant genotypes is the most effective strategy to reduce Begomovirus incidence. Since no commercial cultivars with resistance are currently available, in this study, 15 Capsicum annuum accessions with different levels of domestication were inoculated separately with infectious PepGMV and PHYVV dimers by bioballistics, in order to identify sources of genetic resistance or tolerance to these viruses. Symptom progression (severity), incidence, the area under the disease progress curve (AUDPC), and molecular detection of viral DNA by PCR were recorded in asymptomatic plants. PCR results with oligonucleotides targeting PepGMV and PHYVV showed that 96% of asymptomatic plants were positive, confirming that viral replication occurred without the development of visible symptoms. Significant differences were observed among accessions, with wild and semidomesticated accessions showing very low values of severity, incidence, and AUDPC. Notably, the wild accession Acc-106 exhibited resistance to PepGMV and tolerance to PHYVV, with minimum values of severity (0 and 0.13) and incidence (0% and 13%) at 35 days postinoculation.